Not known Facts About flow cytometer
Not known Facts About flow cytometer
Blog Article
Facts-enriched characterization of mobile populations. Morphological facts from images can incorporate into the richness of flow cytometry information. As an example, the figure shows an or else standard apoptosis assay using Annexin V and PI, introducing mobile imaging to characterize cells in Every population to expose morphologically distinct capabilities. These insights could not are actually attained from multiplex staining alone.
Regardless of whether you’re a beginner or a specialist, check out our methods to progress your analysis and abilities in flow cytometry.
Mix acoustic focusing technological know-how with substantial-parameter Evaluation for adaptable spectral unmixing and standard workflows.
Learn new insights into your experimental info by visualizing populations employing mixed fluorescence and morphology details. Our computer software interface wizards allow downsampling, concatenation (merged files) and dimensionality resources which have been designed in to the software program for an intuitive, streamlined facts Assessment encounter.
"A picture is just not rather really worth one thousand dots, but it surely really helps you to see photos. It is great to find out pictures that correlate with staining. By way of example, AnnexinV-beneficial cells that are morphologically diverse than wholesome cells, or CD14+ monocytes which have been more substantial plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Below, a highly trained user has gated singlets confidently. After analyzing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate is made up of over 4% aggregates.
Pair your Attune flow cytometer with an autosampler for increased workflow performance, and increase robotic integration for maximum throughput.
Our experts will layout panels for you personally as repeatedly you'll need—free of charge, no products obtain desired.
The Attune NxT cytometer is the trusted workhorse instrument your lab can trust in for consistent and dependable general performance. The Attune here CytPix product includes a superior-velocity brightfield digicam and AI-driven impression Assessment software package, with current updates like equipment Mastering applications and an intuitive person interface, enabling Improved facts precision, accuracy, and more rapidly insights into sample biology.
Most likely most of all, these situations incorporate cells of clearly unique phenotypes which may lead to incorrect conclusions relating to double good gatherings (particularly in scarce populations).
AI-pushed automated graphic Examination Using the Attune Cytometric Application translates occasion features into distinct morphology parameters which can be coupled with conventional fluorescence and scatter parameters to reveal very reproducible inhabitants variations and distinctive subsets.
We previously shown the power of imaging Motor vehicle-T/Ramos mobile interactions. Enable’s look at just the population of biggest interest, the double good gatherings, To find out more. We can now use prolonged morphological parameters (right here, circularity and skewness of intensity) to more examine the features of such populations and refine gating on these situations, increasing info robustness.
Leverage the power of Highly developed technological know-how and innovation to accelerate your discoveries. Have faith in within the Attune Flow Cytometer household to deliver the reliability, pace, and precision that established your work apart.
No clean/no lyse leukocyte analysis with imaging. Cells have been obtained from 24-hour-old blood by dilution in one mM EDTA ( Specialist UX/UI allows customers to teach AI styles for expanded applications. (A) The “Train Product” attribute presents annotation applications and genuine-time model schooling accuracy.
Gating on these extended parameters allows you to swiftly and correctly recognize populations of desire and confirm gating strategy with little if any handbook evaluation. The Attune software package delivers usage of point out-of-the-art device Discovering Examination tools inside an expertly intended consumer interface, making it exceptionally simple to operate.
The Attune Xenith cytometer is a higher-parameter spectral analyzer, offering the two spectral unmixing and conventional flow capabilities for comprehensive and versatile mobile Examination.
The Attune CytPix Flow Cytometer allows end users to visualise samples for the duration of acquisition, confirm the accuracy of gating approaches, and support results with reliable documentation for publication needs.
What seemed to be a homogeneous population is actually far more numerous—an Perception that needs to be viewed as when interpreting final results.